Effect of SDF-1α on migration and adhesion of Jurkat T cells.
(A) Transwell assays for cell migration in response to either matrix-presented SDF-1α or SDF-1α added in solution to the bottom chamber (as per the standard assay). Both presented and “soluble” show similar dose responses for stimulation and inhibition of migration. (B) Tops and bottoms of Transwell membranes were first coated with Fn, then with the indicated concentrations of SDF-1α. 106 cells were loaded in the top of each well and allowed to migrate 14 hours, and migrated cells were counted. Values represent mean of 3 trials ±1 SD. (C) Percentage of cells adhering to Fn bound with the indicated concentration of SDF-1α over a range of “spin-off” forces. Presented SDF-1α has no effect on either the strength or the efficiency of Jurkat T-cell adhesion. Values represent mean of 6 trials ±1 SD.
