Fig. 3.
Expression of c-fes transgenes in murine tissues.
(A) Expression of c-fes in the spleen and bone marrow of a nontransgenic CD1 animal and 4 founders generated with the Δ3′ construct. Transgenics containing 1, 30, 32, and 18 copies of Δ3′ DNA were assayed in this experiment. (B) RNase protection analysis of human c-fes mRNA in transgenic tissues harvested from 3 founder mice generated with the Δ6-18a genomic construct (see Figure 2). As described in Figure 1, RNA samples were assayed with both the human- and murine-specific c-fes riboprobes. Transgene copy numbers were determined by Phosphorimager densitometric analysis of Southern blots prepared with transgenic tail DNAs.
