Effect of MIP-1α on monocyte response to fMLF.

Monocytes were preincubated for 1 hour at 37°C with 1 μg/mL MIP-1α. After washing 3 times with PBS, the cells were examined for migration in response to fMLF and MIP-1α (A). *P < .01 compared with response of cells pretreated with medium alone. For calcium mobilization, monocytes were first loaded with Fura-2 and then treated with MIP-1α for 1 hour at 37°C. After washing, the cells were measured for Ca⁺⁺ flux induced by fMLF (B).