American Society of Hematology

Fig. 4.

$Fig. 4. FANCG binding promotes the nuclear accumulation of the FANCA protein. / The indicated isogenic pairs of mutant and corrected lymphoblasts were fractionated into cytoplasmic and nuclear components. A control normal lymphoblast line (PD7) was also included. Protein (100 μg) from each sample was electrophoresed; transferred to nitrocellulose; and immunoblotted with anti-FANCA antiserum, anti-FANCG antiserum, or anti-FANCC antiserum (first 3 panels, respectively). To ensure effective fractionation, the same samples were analyzed for topoisomerase (nuclear) and β-tubulin levels (cytoplasmic). Note that the FANCA antiserum reacts nonspecifically with a protein that runs just above the FANCA band (indicated by an arrow in the FANCA panel), and the antiserum is present in all samples including the HSC72 cells, which do not express any FANCA protein.$

FANCG binding promotes the nuclear accumulation of the FANCA protein.

The indicated isogenic pairs of mutant and corrected lymphoblasts were fractionated into cytoplasmic and nuclear components. A control normal lymphoblast line (PD7) was also included. Protein (100 μg) from each sample was electrophoresed; transferred to nitrocellulose; and immunoblotted with anti-FANCA antiserum, anti-FANCG antiserum, or anti-FANCC antiserum (first 3 panels, respectively). To ensure effective fractionation, the same samples were analyzed for topoisomerase (nuclear) and β-tubulin levels (cytoplasmic). Note that the FANCA antiserum reacts nonspecifically with a protein that runs just above the FANCA band (indicated by an arrow in the FANCA panel), and the antiserum is present in all samples including the HSC72 cells, which do not express any FANCA protein.

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