American Society of Hematology

Fig. 6.

$Fig. 6. Analysis of the subcellular localization of endogenous FANCG protein by cell fractionation. / The FA-A fibroblasts GM6914 were infected with a retrovirus carrying the wild type FANCA cDNA (lanes 5 and 6) or the mutant FANCA-H1110P cDNA (lanes 7 and 8). The parental (uninfected) and infected GM6914 cells, together with a normal control fibroblast line (GM0637, lanes 1 and 2), were fractionated into cytoplasmic and nuclear components. Samples from all fractions were analyzed by immunoblotting with antisera to FANCA and FANCG. To ensure effective fractionation, the same samples were analyzed for topoisomerase (nuclear) and β-tubulin levels (cytoplasmic).$

Analysis of the subcellular localization of endogenous FANCG protein by cell fractionation.

The FA-A fibroblasts GM6914 were infected with a retrovirus carrying the wild type FANCA cDNA (lanes 5 and 6) or the mutant FANCA-H1110P cDNA (lanes 7 and 8). The parental (uninfected) and infected GM6914 cells, together with a normal control fibroblast line (GM0637, lanes 1 and 2), were fractionated into cytoplasmic and nuclear components. Samples from all fractions were analyzed by immunoblotting with antisera to FANCA and FANCG. To ensure effective fractionation, the same samples were analyzed for topoisomerase (nuclear) and β-tubulin levels (cytoplasmic).

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