Surface expression of CXCR-1 and CXCR-2 on PMN at 10-minute preincubation with PBP, CTAP-III, or NAP-2.
PMN (1 × 106/mL) were pretreated for 10 minutes at 37°C with the indicated concentrations of the respective stimulus. Surface-expressed receptors were subsequently immunodetected using the monoclonal antibodies versus CXCR-1, termed SE-2, or versus CXCR-2, termed RII-115, as primary and subsequently GαMig-FITC as secondary antibody as outlined in “Materials and methods.” Under every treatment applied the population stained homogeneously. Background stainings of isotype-matched controls were subtracted. The impact of stimulus preincubation on receptor detection was expressed as a percentage of the median fluorescence observed with PMN stained upon preincubation without the stimulus. Shown are means ± SD of data obtained from 3 independent experiments. ▪, NAP-2; ●, CTAP-III; ▴, PBP.
