CD154 transgene expression in blood mononuclear cells isolated after infusion of autologous Ad-CD154–transduced CLL cells.

To demonstrate CD154 transgene and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GA3PD) expression, RT-PCR was performed on messenger RNA (mRNA) isolated from blood mononuclear cells isolated from patient 003 prior to cell infusion and 0.5, 1, 2, 8, and 24 hours thereafter. The far right lane (+) represents RT-PCR performed with mRNA prepared from an aliquot of Ad-CD154–transduced cells used for infusion.