Fig. 3.
Ex vivo T-cell production of IFN-γ in response to autologous CLL cells before and after infusion of Ad-CD154–transduced CLL cells.
The data from 1 of 3 representative experiments on each of 3 different patients are presented. Blood mononuclear cells were isolated from patient 004 prior to (T-Pre) and 4 weeks after infusion of Ad-CD154–transduced CLL cells (T-Post). T cells were isolated from the blood mononuclear cells using CD4- and CD8-specific Dynabeads. A 48-hour ELISPOT for IFN-γ–producing T cells was performed at a responder/stimulator ratio of 1:1. The mean number of spots per duplicate wells is expressed as the T-cell precursor frequency per 1 × 105 T cells. The error bars indicate the SD about the mean.
