Analysis of polymorphic markers.

Six polymorphic markers (D21S396, D21S258, D21S65, D21S270, D21S49, and D21S1259) are located in 21q11.1, 21q22.11, 21q22.1-q22.2, 21q22.2, 21q22.3, and 21q22.3, respectively. The heterozygosity values are 0.67, 0.76, 0.80, 0.86, 0.72, and > 0.7, respectively. Microsatellite analyses were analyzed in patients 3 to 5 at the time of diagnosis and at complete remission (CR). At diagnosis, LOH was observed in patient 3 for markers D21S65 and D21S270, markers D21S258 and D21S49 were heterozygote, and markers D21S1259 and D21S369 were not informative. In patient 4, only markers D21S369 and D21S258 were heterozygote (markers D21S49 and D21S270 were not informative), and LOH was seen for markers D21S65 and D21S1259. In patient 5, LOH was observed for all the studied markers except D21S369, and D21S258 and D21S49 were informative. Analysis of length of fluorescent fragments was performed on ABI-PRISM 377 P.E. Arrows show the LOH at diagnosis.