EMSA of wild-type and mutant AML1.

³²P-labeled M4 (lanes 1, 2, 4, 6, 8, and 9) or M24 (lanes 3, 5, and 7) was incubated with nuclear extracts containing 30 μg of protein from COS-7 cells transfected with pME18S (lane 1), pME-AML1 (lanes 2, 3, 8, and 9), pME-AML1 V105ter (lanes 4, 5, and 8), or pME-AML1 R139G (lanes 6, 7, and 9). W indicates M4 probe; M, M24 probe. Arrows indicate the shifted bands and free probes.