Fig. 3.
IL-6 and IL-8 protein production in KS Y-1 and KS-59 in response to treatment with 1α,25 dihydroxyvitamin D3.
KS cell lines were seeded at a density of 5 × 104 and treated with the indicated concentrations of 1α,25 dihydroxyvitamin D3. Supernatants were collected after 24 hours and analyzed for IL-6 and IL-8 levels by ELISA. The levels were corrected for the cell number and presented as mean ± SD of 2 separate experiments done in triplicate.