Fig. 3.
Factor Xa–ZPI interaction on alkaline gel electrophoresis.
Mixtures containing various combinations of factor Xa (10 nmol/L), PZ (32 nmol/L), ZPI (28 nmol/L), cephalin (15 μmol/L), and CaCl2 (4 mmol/L) were incubated in HSA at room temperature. After 2 minutes, samples were separated by 8% alkaline-PAGE and analyzed by Western blotting with goat anti–factor Xa antibodies.
