MO7p210 dose response curves for each of the antileukemic agents alone and in combination with 0.05μmol/L STI571.
Cells were analyzed daily for uptake of MTT in the presence or absence of various antileukemic agents. Data from day 3 is plotted as the concentration of antileukemic drug versus inhibition of proliferation, comparing each antileukemic agent alone (⋄) to each antileukemic agent with 0.05 μmol/L STI571 (▴). Each data point represents a mean across 4 identical wells from 1 of 3 separate experiments. Standard deviations ranged from ± 0% to 13%, with a median of 2%. Regression lines generated in Microsoft Excel represent the best fit relationship between drug concentration and the percentage of inhibition of proliferation. R2 values indicate the percentage of data that can be accounted for by the regression line. IC60 concentrations for the MO7p210 with each antileukemic alone and in combination with STI571 were determined from these graphs and are listed in Table 1. Identical analyses were performed for both the MO7e and K562 cell lines, with the results summarized in Table 1.
