Analysis of αGal epitope expression in lymphoid tissues.

Analysis of αGal expression in lymphoid tissues of mice reconstituted with either LGTA7- (column A) or NEO^r- (column B) transduced bone marrow 18 weeks after bone marrow transplantation by staining with IB4 lectin, followed by flow cytometry. Flow cytometry analysis gates were set as described in Figure 2. Expression of αGal epitopes in lymphoid tissues of secondary mice reconstituted with either LGTA7- (column C) or NEO^r- (column D) transduced bone marrow 22 weeks after secondary bone marrow transplantation. Shown are the results from representative mice.