GT⁰ mice reconstituted with LGTA7-transduced bone marrow are tolerant to αGal.

Panel A: analysis of αGal reactive IgM serum antibodies in mice reconstituted with either LGTA7- (▪, n = 9), or NEO^r-transduced bone marrow (●, n = 9) after immunization with pPBMCs by ELISA. Shown are the mean and standard deviation of combined data from 2 independent experiments. Control GT⁰ (○) and GT⁺ (▵) mice immunized at the same time that did not receive a bone marrow transplant are shown for comparison purposes. Panel B: analysis of splenic anti-Gal–producing B-cell frequency by ELISPOT. Shown are values obtained for immunized GT⁺ mice (░) and GT⁰ control mice (▨), and mice receiving LGTA7- (■), or NEO^r-transduced bone marrow (▪) after immunization. Shown are the mean and standard deviation values for each group. All samples were assayed in duplicate.