Fig. 3.
Inhibition of capillary-like tube formation by uPAR antibodies.
hMVECs were cultured on top of a 3-dimensional fibrin matrix in M199 supplemented with 10% HS and 10% NBCS under normoxic or hypoxic culture conditions and were stimulated with 10 ng/mL each FGF-2+TNF-α in the presence of 100 μg/mL pAb anti-tPA, 100 μg/mL pAb anti-uPA, 5 μg/mL mAb H-2 (anti-uPAR), or 100 U/mL aprotinin. After 3 days of culturing, the mean tube length (mm/cm2) was measured as described and expressed as a percentage of FGF-2+TNF-α. The data represent the mean plus or minus SD of 3 independent experiments performed in duplicate wells.
