Hypoxia increases uPAR antigen levels.

hMVECs were cultured for 72 hours in normoxic and hypoxic conditions in M199 supplemented with 10% HS and not stimulated (control) or stimulated with 10 ng/mL each FGF-2+TNF-α or with 10⁻⁸mol/L phorbol myristate acetate (PMA). Subsequently, the cells were cooled on ice, and the specific binding of ¹²⁵I-labeled DIP-uPA to hMVECs was determined and expressed as fmol¹²⁵I-uPA/10⁵ cells. The data represent the mean plus or minus SEM of 4 independent experiments performed in duplicate wells. The asterisk indicates P < .05, which is significantly different from the normoxic counterpart.