Fig. 6.
Hypoxia does not decrease uPA production.
hMVECs were cultured for 72 hours in normoxic and hypoxic conditions in M199 supplemented with 10% HS and not stimulated (control) or stimulated with 10 ng/mL each FGF-2+TNF-α in the presence of 0 or 5 μg/mL mAb H-2. After the incubation period, the uPA antigen levels were determined in the conditioned media by ELISA, as previously described, and expressed as ng uPA/105 cells. The data represent the mean plus or minus SD of 3 independent experiments performed in duplicate wells. The asterisk indicates P < .05, which is significantly different from the normoxic counterpart.
