Plasmin formation is increased in hypoxia.

hMVECs were cultured in M199 supplemented with 10% HS and 10% NBCS and not stimulated (ns) or stimulated with 10 ng/mL each FGF-2+TNF-α in normoxia (bT/normoxia) or hypoxia (bT/hypoxia). Normoxic hMVECs were cultured in the presence of 5 μg/mL mAb H-2 or 200 U aprotinin. After 72 hours the plasmin formation was measured as described in “Materials and methods.” H-2 and aprotinin were also present during the plasmin formation assay. The data are expressed as the mean plus or minus SD. Each condition was performed 8-fold. The asterisk indicatesP < .002, which is significantly different from normoxic FGF-2/TNF-α–stimulated cells.