Southern blot analysis of reverse transcriptase–polymerase chain reactions (RT-PCRs) for the gene transcripts corresponding to G3PDH, LL-37, and HNP 1-3.
The amplified bands were the expected sizes, and the identities of these bands were confirmed by hybridization with phosphorus 32 (32P)–labeled radioactive probes. (A) Loaded material was from the different NK-cell clones indicated on top of each lane; the complementary DNA identity is indicated on the left. One NK-cell clone was stimulated with bacteria. (B) The origin of the loaded material is as follows. BL-28, CIR, and JY are of B-cell origin. Jurkat, J1 T cells, Molt 16, and Hut 68 are derived from αβ T cells. U937 is a monocytic cell line, and SE δ1/δ3, GN PBL γδ, and D768/6 (Vγ3/Vγ9) are of γδ T-cell origin.
