Expression of LL-37 in stimulated lymphocytes.

(A) Western blot analyses for the presence of LL-37 in supernatants from enriched peripheral blood mononuclear cells (PBMC) and T and NK cells. The 2 cell groups were grown and stimulated with IL-6, interferon γ, or both for 6, 15, and 24 hours (indicated on right). The origin of the loaded material is shown on the top of each lane. The results were obtained with use of an electrogenerated chemiluminescence detection system. (B) RT-PCR and Southern blot analyses for expression of G3PDH and LL-37. The cells used for expression analyses at the transcriptional level were the same as those used in the studies shown in A.