HLA tetramers complexed with EBV epitopes stain EBV-specific CD8 T cells.

EBV-specific T cell lines were generated from EBV⁺individuals AD and EP by in vitro restimulation with autologous B-LCLs, as described in “Materials and methods.” HLA-A andHLA-B alleles expressed in these individuals and the HLA tetramer used for staining are indicated above each histogram. Each plot is gated on live, CD8⁺ T lymphocytes, and the percentage of tetramer-positive T cells is indicated in the right upper corner of each histogram.