Serial analysis of PBMCs following allo-PBSCT reveals that EBV-specific T-cell populations reconstitute the periphery rapidly and fluctuate in frequency.

PBMCs were obtained 30, 60, 90, and 120 days after allo-PBSCT from patient C, who expresses both HLA-A2 and HLA-B8, and stained as described in Figures 2 and 3A. The percentage of live CD8-gated T cells that stained with the respective tetramers is indicated in the upper quadrants of each dot-plot.