Fig. 3.
![Fig. 3. RA-induced conformational changes in wild-type and mutant PML/RARα fusion proteins. / Limited trypsin digestion analysis of wild-type and mutant PML/RARα S-form (A) and L-form (B). In vitro [35S]-methionine synthesized PML/RARα proteins were incubated without (−) or with (+) 1 μmol/L RA, and subsequently treated with increasing trypsin concentrations (0 to 25 μg/mL). Digestion products were analyzed by denaturing electrophoresis. The arrows indicate the intact PML/RARα proteins. Asterisks indicate resistant fragments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/9/10.1182_blood.v96.9.3200/5/h82100300003.jpeg?Expires=1735840133&Signature=kQEjDBC~Ve7gssPIOpiOJlawJYplFbAdrUa1WGxTrvur2cXpFCrSLbbsIZq-SJYl24tsuK9-RxLUnLhsbESRxJ4FleNO07r0Yfecz6EQfeTQBrz3mG2yMMMdAcLvct~iEnTOnJbFPD0U0hIhtNwxJLEIJlTVSNrlHkoVWF1AcOQ1q8l~2OxAURv-nc3Xn9S6DE~Iqv-YD0BlPCCFSy2Z909QYSBy9DWHNfp9~uXE2hcBFKHBYYmumhLTTup6iESd6VJU1Oza1PHbFMRDcc7eylM3FRWIYyAlJ7GU0Dbj1-L-~NFGBvTnvRj1cqOR3ktA6mwkCPj5VMs49ehQQ2uXdQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RA-induced conformational changes in wild-type and mutant PML/RARα fusion proteins.
Limited trypsin digestion analysis of wild-type and mutant PML/RARα S-form (A) and L-form (B). In vitro [35S]-methionine synthesized PML/RARα proteins were incubated without (−) or with (+) 1 μmol/L RA, and subsequently treated with increasing trypsin concentrations (0 to 25 μg/mL). Digestion products were analyzed by denaturing electrophoresis. The arrows indicate the intact PML/RARα proteins. Asterisks indicate resistant fragments.
