Transcriptional activity of wild-type and mutant PML/RARα fusion proteins.

DR5-tk-CAT (A) or TREpal-tk-CAT (B) reporters were cotransfected with the indicated PML/RARα. The pCMX and the pSG5 represent the vectors alone. Relative CAT activity without (control) or with indicated concentrations of RA is shown, with the calculated corresponding fold induction below. Each data point represents results from at least 3 independent transfections. Control, ▪; RA 0.01 μmol/L, ▧; RA  0.1 μmol/L, ▤; RA 1 μmol/L, ▨.