Fig. 5.
![Fig. 5. Ligand-dependent dissociation of the corepressor SMRT from wild-type and mutant PML/RARα fusion proteins on a DR5 RARE in gel mobility shift assay. / Interaction of SMRT with wild-type and mutant PML/RARα S-form (A) and L-form (B). In vitro translated PML/RARα fusion proteins were coincubated with the [32P]-labeled DR5 RARE, along with bacterially expressed GST-SMRT-IDII in the presence of increasing concentrations of RA. The position of the complex shifted by SMRT is indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/9/10.1182_blood.v96.9.3200/5/h82100300005.jpeg?Expires=1735839137&Signature=KJED9-M1l8~-wO9Jtxp7pBtTyjQi8cIvp7N5wuGwMbKIAD9XhUBOlgAs4RpKsjSroKDxBov8FK94IHxKyJoxe5Xjn~drr2zvi6Xh7-NfdM4PuuKXAfFYdx6oi3SFggq-ziq3pwau6sTQLK7A6yKADQF4CYVUVcwpwW2uPe7Vr7czrBJJtSK4tHUqtQumMr-q3AFKTwQhnxBxR7M2mh7zC7C3xARgKZDOKV7Bfec2mJjIQw5m4IyqTcONVJZXs2raIh-NpBv30pgpb0dfTdYA5rjookgiNKCcF62Nz7O2Tvh5BRPP7JY0HIY6Lmq1rDti0w-sI8V4ccWvFQTA6Qv2pg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ligand-dependent dissociation of the corepressor SMRT from wild-type and mutant PML/RARα fusion proteins on a DR5 RARE in gel mobility shift assay.
Interaction of SMRT with wild-type and mutant PML/RARα S-form (A) and L-form (B). In vitro translated PML/RARα fusion proteins were coincubated with the [32P]-labeled DR5 RARE, along with bacterially expressed GST-SMRT-IDII in the presence of increasing concentrations of RA. The position of the complex shifted by SMRT is indicated.
