Fig. 3.
IFN-α1 mRNA cannot be detected after ATRA stimulation of U-937 cells.
U-937 cells were stimulated by ATRA for 48 hours, total RNA was prepared, and cDNA was synthesized by RT-PCR. PCR was run with cDNA preparations from untreated (lanes 1, 4, 7) or ATRA-stimulated cells (lanes 2, 5, 8) with primers specific for IFN-α1 (lanes 1-3), Stat1 (lanes 4-6), or β-actin (lanes 7, 8). To test for genomic contamination, PCR was also run with preparations of mRNA without reverse transcriptase, from untreated (lane 9) or ATRA-stimulated (lane 10) U-937 cells, using β-actin primers. Positive controls were lanes 3 and 6.
