Fig. 4.
Stat1HAY701F inhibits ATRA-induced GBP-Luc reporter activity.
U-937 cells were transiently cotransfected with 0.2 μg of GBP-luciferase reporter ± 0.75 μg of pCI-Stat1, pCI-Stat1Y701F, or empty pCIneo vector, as indicated. At 12 hours after induction the cells were induced by 1 μmol/L ATRA for 36 hours after which extracts were prepared and the luciferase activity measured. Differences in transfection efficiency were corrected for by cotransfection of β-actin–LacZ and measurement of β-galactosidase activity. Data shown are the average of duplicates.
