ATRA-induced expression of differentiation markers CD11c and G-CSFR is repressed by Stat1HAY701F-expressing U-937 cells.
(A, B) Induction of CD11c (A) and G-CSFR (B) expression by ATRA treatment in U-937 sublines. Cells were induced by ATRA, collected at the indicated time points, and analyzed by flow cytometry. Data are presented as mean fluorescence intensity (MFI) values correlated to autofluorescence of unlabeled cells. Mean values ± SD (n = 5). The asterisks indicate significant differences to the corresponding pCIneo controls (P < .05). (C) ATRA-induced expression of IRF-1 is not inhibited in U-937 sublines. Total RNA was prepared from unstimulated cells or cells treated with ATRA for 12 and 72 hours, and subjected to Northern blot analysis with IRF-1 and GAPDH probes.
