Up-regulation of DAF expression on HUVECs following stimulation with thrombin.

The expression of DAF on resting and thrombin-stimulated HUVECs was assessed by flow cytometry using MoAb 1H4. The figure shows background fluorescence (FITC-labeled rabbit antimouse antibody alone) (shaded histograms) and DAF expression (open histograms) on unstimulated HUVECs (A) and HUVECs stimulated with thrombin (10 U/mL) for 6 hours (B), 12 hours (C), 24 hours (D), 48 hours (E), and 72 hours (F). The RFI for DAF expression at each time point is shown in brackets. The RFI represents the mean MFI with the test MoAb divided by the MFI using an isotype-matched irrelevant MoAb. The results are representative of 3 experiments on separate EC cultures.