Fig. 2.
Fig. 2. Dose response for thrombin-induced DAF and ICAM-1 expression. / HUVEC monolayers were incubated for 24 hours in the presence or absence of increasing concentrations of thrombin before harvesting and analysis by flow cytometry. DAF and ICAM-1 were detected using MoAbs 1H4 and 6.5B5, respectively. The results are expressed as mean ± SEM for RFI DAF (A) and ICAM-1 (B). The results are representative of 3 separate experiments performed in triplicate wells using different EC lines.

Dose response for thrombin-induced DAF and ICAM-1 expression.

HUVEC monolayers were incubated for 24 hours in the presence or absence of increasing concentrations of thrombin before harvesting and analysis by flow cytometry. DAF and ICAM-1 were detected using MoAbs 1H4 and 6.5B5, respectively. The results are expressed as mean ± SEM for RFI DAF (A) and ICAM-1 (B). The results are representative of 3 separate experiments performed in triplicate wells using different EC lines.

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