Analysis by confocal microscopy of F-actin and CD44 in HCs on HA with or without IL-8.

HCs adherent to HA were stained with rhodamine-phalloidin (for F-actin) or with an anti-CD44 MoAb. In the absence of IL-8, both F-actin and HA showed a polarized distribution in many cells, whereas few or no such polarized cells were observed in the presence of IL-8 (representative examples of 6 separate experiments).