Effect of IL-8 on F-actin polymerization in HCs.

HCs were incubated with or without IL-8, and polymerized F-actin was measured by FACS analysis of fixed and permeabilized cells stained with rhodamine-phalloidin. (A) Representative example of 3 experiments with cells from 3 patients. The intensity of staining varied but was similarly enhanced by IL-8 in all instances. (B) Two representative FACS histograms of F-actin in unstimulated or IL-8–stimulated cells on HA after 30 minutes of incubation. Note the presence of 2 distinct populations and the recruitment of cells to the F-actin–bright population after IL-8 stimulation.