Mildly oxidized LDL can induce surface tissue factor pathway activity.

Cells in DMEM or DME/F12 were treated with LDL (200 μg protein/mL) for 6 hours before assessment of tissue factor pathway activity. Cells were pretreated with solvent (control, ■) or ebselen (10 μmol/L, ▪) overnight before and during the addition of LDL. Data represent fold induction over DMEM, lipoprotein-free control. DMEM and DME/F12 lipoprotein-free controls yielded identical tissue factor pathway activity. Data represent means ± SD of 4 wells per treatment.