Alignment of the nucleotide sequences and genotyping of the internal repeat domain detected in the KS lesions.

(A) Alignment of the nucleotide sequences of the 2 highly variable regions (VR1 and VR2) of the orf-K1 gene. The alignment includes a prototype K1 sequence (clade A) from the BC-1 PEL cell line (GeneBank accession number U75698), and the K1 sequences (clade C) detected in the KS lesions from each of the 2 renal recipients (Pt 1 and Pt 2), after transplantation. Dots indicate identity with the first sequence in the alignment. (B) Genotyping of the polymorphic internal repeat domain of orf-73 gene detected in the KS lesions from each of the 2 renal recipients (Pt 1 and Pt 2), after transplantation, in the cutaneous KS lesion from an elderly Italian patient with classic KS (KS), and in the pleural PEL specimen from an elderly HIV negative Italian patient (PEL). nc, negative control, represented by the DNA from HHV-8 negative Raji cell line; mk VII, molecular weight marker (Boehringer Mannheim, Germany).