Fig. 5.
![Fig. 5. Enhancement of C/EBPε mRNA expression in NB4 cells by indinavir. / NB4 cells were cultured for 48 hours under various conditions: lane 1, (diluent control); lane 2, indinavir (2 × 10−5 mol/L); lane 3, ATRA (10−9 mol/L); lane 4, combination of indinavir (2 × 10−5 mol/L) and ATRA (10−9mol/L). Total RNA was extracted and analyzed by Northern blot technique (30 μg/lane) and hybridized with [32P]-labeled C/EBPε cDNA probe as described in “Materials and methods.” The same blot was rehybridized with [32P]-labeled GAPDH probe to show equality of RNA loading in each lane. Results are expressed as fold increase in expression as compared with control NB4 cells (lane 1). The band intensity was measured using a densinometer.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/96/10/10.1182_blood.v96.10.3553/5/h82200383005.jpeg?Expires=1735505589&Signature=vXMYDn-pM0gPr-ZhlHMsEF4qFTs9E5-ftgzli319XEAlJ9cN3NLLaoybu7PioMjxZMw939JMfpJfFtHfjBq7kV8EbA9tHmHqmDyr~AxCZ8XJPycBwSS3~zatcN4R8zP5LEqVrzegCqFOxdeqF2OVmwgaGvDoSxp2aRyCtzmXELdrqgzeD~KfT2AQKAjT0ujiginCBpEyIJeUdiEL0~pS8KculJ2PEkw7CyzxT~FnC75NhQiYW0syOzx39YpdvGhonKOrApuZehqz8fl30vymiCPbAQZkWsUiDLjJ7DfBDlxt2rBTZ8crj0c1wWvpOFVm5O8GveQHksvzRB7OcuWvAQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Enhancement of C/EBPε mRNA expression in NB4 cells by indinavir.
NB4 cells were cultured for 48 hours under various conditions: lane 1, (diluent control); lane 2, indinavir (2 × 10−5 mol/L); lane 3, ATRA (10−9 mol/L); lane 4, combination of indinavir (2 × 10−5 mol/L) and ATRA (10−9mol/L). Total RNA was extracted and analyzed by Northern blot technique (30 μg/lane) and hybridized with [32P]-labeled C/EBPε cDNA probe as described in “Materials and methods.” The same blot was rehybridized with [32P]-labeled GAPDH probe to show equality of RNA loading in each lane. Results are expressed as fold increase in expression as compared with control NB4 cells (lane 1). The band intensity was measured using a densinometer.
