IFN-γ secretion and CD69 up-regulation in CD8 T cells from representative subject 307 in response to stimulation with HIV-infected CD4 T cells is enhanced in the presence of IL-2.

Immunomagnetically isolated CD8⁺ T cells were stimulated with uniformly HIV-infected CD4 T cells in the presence (D) or absence (B) of 600 IU/mL of IL-2. Cultures stimulated with uninfected CD4 blasts in the presence (C) or absence (A) of IL-2 served as controls. Cells were incubated overnight in the presence of Brefeldin A and then stained externally for CD8-Cy5 and CD69-PE and internally for IFN-γ FITC.