CLHb enhanced the aggregation of rabbit platelets stimulated with collagen.

Impedance aggregometry was used to evaluate the effect ofo-raffinose CLHb on platelet aggregation in rabbit whole blood. CLHb enhanced the aggregation of platelets stimulated with collagen (1 μg/mL) in a dose-dependent fashion as shown in a representative experiment (A) and results of assessment of aggregation amplitudes at 6 minutes in 15 experiments (B). The change in platelet aggregation (Δ aggregation) was calculated as the aggregation in the presence of CLHb minus that observed in Ringer's lactate alone. The data are expressed as means; error bars denote 1 SD. *P = .01 and **P < .001 compared with results with Ringer's lactate alone.