Expression of the MEL1 gene in mRNA from leukemia cells with t(1;3)(p36;q21) by Northern hybridization.

The MEL1 transcript (8 kb) was expressed in leukemia cells with t(1;3)(p36;q21) (lane 5). However, the MEL1 gene did not express in other leukemia cell lines, HUT78 (lane 1), MOLT3 (lane 2), Kasumi-3 (lane 3), and UCSD/AML1 (lane 4). The same membrane was hybridized to EVI1 or β-actin probes, sequentially.EVI1 transcripts (6 kb) were expressed in both Kasumi-3 with t(3;7)(q26;q22) (lane 3) and UCSD/AML1 with t(3;3)(q21;q26) (lane 4).