Expression of MT1-MMP, MMP2, and TIMP2 mRNA transcripts in platelets.

Using RT-PCR analysis, the expression of MT1-MMP, MMP2, and TIMP2 mRNA transcripts was studied in washed platelets and compared with corresponding mRNA transcripts expressed by human mesangial cells. PCR products stained with ethidium bromide were electrophoresed in a 1.5% agarose gel. The figure shows RT-PCR products of total RNA derived from platelets (Pl) and mesangial cells (Mes) using specific primers for MT1-MMP, MMP2, and TIMP2. Left and right panels represent the molecular size markers, and the bands of 550, 620, and 540 base pairs correspond to MT1-MMP, MMP2, and TIMP2, respectively.