Fig. 2.
Exon 8 and 9 IGF-2 RT-PCR products (primers 68 and 69) from 5 representative heterozygous bone marrow RNA samples digested with ApaI orAvaII.
All show biallelic transcription of IGF-2. The use of bothApaI (A) and AvaII (B) excludes misinterpretation from partial enzyme digestion, whereas the product size excludes DNA contamination (Figure 1). Lane M shows λDNA digested withHindIII and EcoRI. All gels shown contain 2% agarose gel with ethidium bromide.