Detection of sMUC1 release in MM cell line culture supernatants using the CA27.29 immunoassay.

CA27.29⁺ RPMI 8226 and S6B45 MM cells; CA27.29⁺ZR-75-1 breast cancer cells, and CA27.29^-HS Sultan MM cells were cultured in human plasma. Supernatants were collected after 48 hours of culture, and sMUC1 levels determined using the CA27.29 immunoassay. Human plasma from the same lots was used as control supernatants to determine baseline sMUC1 levels. Experiments were performed in triplicate, and standard deviation bars are displayed. *P < .002 versus sMUC1 levels of control supernatants;^#P < .03 versus sMUC1 levels in HS Sultan cell culture supernatants.