CKLiK is homologous to CaMKI and highly expressed in human granulocytes.

(A) Alignment of CKLiK with CaMKI. Residues identical to CaMKI are indicated by an asterisk (*) and gaps in the alignment are indicated with a dash (−).The amino acid sequence is indicated on the right. (B) Distribution of CKLiK was determined by RNase protection. The PCR product encoding the catalytic domain of CKLiK was used to generate a radiolabeled RNA probe. RNA isolated from primary hematopoietic cells and cell lines were hybridized for CKLiK expression. A GAPDH probe was used as a control. Lanes 1 to 3 represent primary leukocytes (1, lymphocytes; 2, monocytes; 3, PMNs) and lanes 4 to 6 represent lymphoid and myeloid cell lines as indicated. (C) Expression of CKLiK in cord blood-derived CD34⁺ stem cells during differentiation toward neutrophils was analyzed by RNase protection as in panel B. Time points indicate the number of days differentiated toward neutrophilic lineage in the presence of G-CSF.