Effect of an anti-β2-integrins (CD18) mAb (TA-4) on eosinophil recruitment into rat pleural cavities.
In these experiments, 3 groups of animals were treated intravenously with saline, control mouse IgG (P1.17; 3.5 mg/kg) or anti-CD18 (TA-4; 3.5 mg/kg). After 15 minutes, the animals were injected intrapleurally with RPMI (100 μL/cavity) or IL-4 (5000 units/100 μL per cavity). Eosinophil accumulation was determined 24 hours later. The results are expressed as the mean ± SEM value for 4 to 7 animals per group. A significant increase (P < .001) over levels in RPMI-treated rats is indicated by 2 asterisks. A significant difference (P < .001) between responses in animals treated with the control antibody and those treated with anti-CD18 mAb is indicated by 2 + symbols.
