Effect of Thal and analogs on cell cycle profile of MM cell lines and patient MM cells.

(A) MM.1S cells, Hs Sultan cells, and patient MM cells were cultured with 10 μmol/L of Thal or 1 μmol/L of IMiD1, IMiD2, or IMiD3 for 72 hours. Cultures in media control alone served as a negative control and 18-hour cultures with 10 μmol/L Dex as positive controls. Cells were then stained with PI, and cell cycle profile was determined by flow cytometric analysis. (B) These MM.1S (▪), Hs Sultan (▧), and patient MM (■) cells were also stained with annexin V as an additional assay for apoptosis.