Internal deletion of several exons within PTEN in OPM2 and Δ47 MM lines.

(A) The RNA isolated from each cell line was reverse transcribed to cDNA and amplified using primers flanking the translation start and stop codons of human PTEN cDNA. The RNA from 7.2 line was also subjected to the 1-step RT-PCR without reverse transcriptase and shown as a negative control (NC). (B) The RT-PCR products shown in panel A were subcloned to a TOPO TA cloning vector. The minipreparation of plasmid DNA was digested with EcoRI and run on 1% agarose gel. Both vector and PTEN inserts are shown after ethidium bromide staining. (C) Deletion of exons 3 to 5 in Δ47-l, 3 to 6 in Δ47-s and 3 to 7 in OPM2 are shown after sequencing analysis.