Forced expression of PTEN in OPM2 and Δ47 results in suppression of Akt activity.

(A) The human and mouse plasma cell tumor lines infected with PTENWT or C124S mutant retrovirus were immunoprecipitated with anti-PTEN. Transferred proteins were immunoblotted with anti-Flag MoAb. Lanes 1, 4, and 7 represent vector-infected cells. Lanes 2, 5, and 8 indicate PTENWT infectants, and lanes 3, 6, and 9 are obtained from PTENC124S infection. (B) Cell lines were treated similarly as in Figure 2A and subjected to an Akt activity assay. Phosphorylated Histone H2B is indicated. (C) Similar treated cell lines as shown in panel B were immunobloted with anti–P-Akt (Ser473).