PTEN is dispensable for tumorigenicity of a mouse plasma cell tumor line.

(A) Scheme of the retroviral infection of 7.2 raf/myc line with the various PTEN constructs is shown. (B) Various infectants were immunoprecipitated (IP) with anti-PTEN and transferred proteins were immunoblotted with anti-Flag (left panel). The same Immobilon P was reblotted with anti-PTEN (right panel). (C) Akt activity assay was performed as described in Figure 2 by using Histone H2B as a substrate. The Δ47 line was used as a positive control. (D) Cell lysates from tumors induced on injecting 7.2 (lane 1), 7.2/vector (lane 2), 7.2/PTENWT (lane 3), and 7.2/PTENC124S (lane 4) were immunoblotted with anti-PTEN.