PCR-based amelogenin gene amplification.

Marrow stromal cells from 2 female patients who received allografts from male donors (case nos. 10 and 14) were generated in vitro, extensively depleted of monocyte-macrophages, and analyzed by PCR for the presence of donor-derived mesenchymal cells. In both cases, a 788 fragment located on the Y chromosome and a 977 fragment located on the X chromosome were detected, thus demonstrating the partial male origin of stromal cells.