Fig. 2.
Cell output from FL versus SCF-supplemented low–cell-density BM cultures in the presence of titrated BM cells with the use of transwell plates.
BM cells at low density (2 × 105 cells per milliliter) were cultured in the lower chambers of transwell plates, separated by a 0.4-μm filter from the titrated feeder cells in the upper chambers. Cells in the upper and lower chambers were cultured in either culture medium alone (▪), 100 ng/mL of FL (▴), or 100 ng/mL SCF (●) for 10 days and then harvested from the lower chamber, counted, and analyzed for expression of cell-surface antigens by flow cytometry. Results shown are representative of 3 experiments.
